containing the disrupted gene
The isolation of mouse ES cells with a gene-targeted
disruption requires positive and a negative
selection. A bacterial gene conferring resistance
to neomycin (neoR) is added (2) into
DNA cloned from the target gene (1). DNA containing
the thymidine kinase gene (tk+) from
herpes simplex virus is then also added to the
vector outside the region of homology. These
two selectable modifications (neoR and tk+) are
part of the replacement vector (3). The vector is
introduced into ES cells, which are grownin culture.
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