The multiresistant plasmid pTP10

A large, 51409 bp plasmid from the multiresistant
Gram-positive Corynebacterium striatum
M82B containing genes encoding proteins
renders its host bacteria resistant to 16 antimicrobial
agents from six structural classes
(Tauch et al., 2000). This is the largest plasmid
to have been sequenced to date. It contains DNA
segments from a plasmid-encoded erythromycin
(Em, shown inside the circular genome diagram)
resistance region from the human pathogen
Corynebacterium diphtheriae, a chromosomal
DNA region from Mycobacterium tuberculosis
containing tetracycline (Tc) and oxacillin
resistance, a plasmid-encoded chloramphenicol
(Cm) resistance region from the soil bacterium
Corynebacterium glutamicum, and a
plasmid-encoded aminoglycoside resistance to
kanamycin (Km), neomycin, lividomycin, paramomycin,
and ribostamycin from the fish
pathogen Pasteurella piscicida. In addition, the
plasmid contains five transposons and four insertion
sequences (IS1249, IS1513, IS1250, and
IS26) at eight different sites. Altogether eight
genetically distinct DNA segments of different